Journal: Cell communication and signaling : CCS

Article Title: Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells.

doi: 10.1186/s12964-019-0324-8

Figure Lengend Snippet: Fig. 5 Oroxylin A inhibition of LDH-A led to blockade of aerobic glycolysis in HSCs. LX2 cells were treated with oroxylin A and/or galloflavin at indicated concentrations, or transfected with LDH-A siRNA for 24 h. a Measurements of glucose uptake. b Measurements of glucose consumption indicated by GOD activity. c Measurements of intracellular lactate levels. d Real-time PCR analyses of mRNA expression of HK2, PFK1 and PKM2. e Western blot analyses of protein expression of HK2, PFK1 and PKM2 with quantification. f Measurements of intracellular HK2 enzyme activity. g Measurements of intracellular PFK1 enzyme activity. h Measurements of intracellular PKM2 enzyme activity. i Real-time PCR analyses of mRNA expression of LDH-A. j Western blot analyses of protein expression of LDH-A with quantification. k Real-time PCR analyses of mRNA expression of HK2, PFK1 and PKM2. For statistical significance of panels a-h: *p < 0.05 vs. control, **p < 0.01 vs. control, ***p < 0.001 vs. control. For statistical significance of panels i-k: *p < 0.05 vs. control siRNA, **p < 0.01 vs. control siRNA, ***p < 0.001 vs. control siRNA

Article Snippet: LDH-A siRNA (sc-43,893) and control siRNA (sc-37,007) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Inhibition, Transfection, Activity Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Cell communication and signaling : CCS

Article Title: Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells.

doi: 10.1186/s12964-019-0324-8

Figure Lengend Snippet: Fig. 6 Oroxylin A inhibition of LDH-A led to reduction of HSC contraction. LX2 cells were treated with oroxylin A and/or galloflavin at indicated concentrations, or transfected with LDH-A siRNA for 24 h. a Collagen gel contraction assays with quantification. b Cytoskeleton fluorescence staining, scale bar: 20 μm. c Western blot analyses of MLC2 phosphorylation with quantification. d Immunofluorescence analyses of MLC2 phosphorylation, scale bar: 20 μm. e Cytoskeleton fluorescence staining, scale bar: 20 μm. f Western blot analyses of MLC2 phosphorylation with quantification. For statistical significance of panels a-d: *p < 0.05 vs. control, **p < 0.01 vs. control. For statistical significance of panels e-f: **p < 0.01 vs. control siRNA

Article Snippet: LDH-A siRNA (sc-43,893) and control siRNA (sc-37,007) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Inhibition, Transfection, Fluorescence, Staining, Western Blot, Phospho-proteomics, Immunofluorescence, Control

Journal: Journal of Clinical Medicine

Article Title: Transscleral Fixation of Black Diaphragm Intraocular Lens in Complete Aniridia and Aphakia Due to Posttraumatic Eye Rupture: A Pilot Study

doi: 10.3390/jcm8010046

Figure Lengend Snippet: Status of 14 patients monitored after black-diaphragm intraocular lens (BD IOL) implantation: gender, age, initial and final best-corrected visual acuity (BCVA), postoperative intraocular pressure, ocular comorbidities, surgical procedure, type of BD IOL, postoperative complications and secondary interventions. Abbreviations: PPV-pars plana vitrectomy, PKP-penetrating keratoplasty.

Article Snippet: 10 , male , 43 , 30 , 0.1 , 0.1 , 12 , corneal scar , BD IOL scleral fixation + anterior vitrectomy , 76 G , non , None.

Techniques:

Journal: Cell calcium

Article Title: The Roles of Orai and Stim in Bone Health and Disease

doi: 10.1016/j.ceca.2019.06.001

Figure Lengend Snippet: Diagram showing expression of Stim and Orai1 proteins in osteoblasts and osteoclasts, and functional consequences of this expression.

Article Snippet: Using the RAW264.7 cells line, Hwang and Putney demonstrated that shRNA knockdown of Orai1 inhibited RANKL-stimulated formation of multinucleated osteoclasts; this effect was confirmed in primary human osteoclast precursors transiently transfected with Orai1 siRNA [ 43 ].

Techniques: Expressing, Functional Assay